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KMID : 1007520220310020231
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Food Science and Biotechnology
2022 Volume.31 No. 2 p.231 ~ p.241
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Human gut commensal bacterium Ruminococcus species FMB-CY1 completely degrades the granules of resistant starch
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Hong Yeong-Sik
Jung Dong-Hyun
Chung Won-Hyong
Nam Young-Do
Kim Ye-Jin
Seo Dong-Ho
Park Cheon-Seok
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Abstract
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Resistant starch (RS) in the diet reaches the large intestine and is fermented by the gut microbiota, providing beneficial effects on human health. The human gut bacterium FMB-CY1 was isolated and identified as a new species closest to Ruminococcus bromii. Ruminococcus sp. FMB-CY1 completely degraded RS including commercial RS types 2, 3, and 4, and generated glucose and maltose; however, it did not assimilate glucose. Genome analysis revealed 15 amylolytic enzymes (Amy) present in FMB-CY1. The evolutionary trees revealed that the Amys were well divided each other. All Amys (4, 9, 10, 12, and 16) containing cohesin and/or dockerin and scaffolding proteins known to be involved in constituting the amylosome, were identified. A new species of Ruminococcus, strain FMB-CY1, was considered to have the ability to form amylosomes for the degradation of RS. This new RS-degrading Ruminococcus species provides insights into the mechanism(s) underlying RS degradation in the human gut.
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KEYWORD
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Amylosome, Gut microbiota, Resistant starch, Ruminococcus, Starch granule
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